Meeting Banner
Abstract #0598

in vivo Non-Invasive Measurement of Altered Hepatic Glutathione Concentration and Synthesis Rate in Acute and Chronic Models of Liver Oxidative Stress

Peter E. Thelwall1, 2, John Skamarauskas1, 2, Daniel Vidler, 23, Michael Dunn, 23, Fiona Oakley2, Michael P. Gamcsik4

1Newcastle Magnetic Resonance Centre, Newcastle University, Newcastle upon Tyne, Tyne and Wear, United Kingdom; 2Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, Tyne and Wear, United Kingdom; 3Medical Toxicology Centre, Newcastle University, Newcastle upon Tyne, Tyne and Wear, United Kingdom; 4Joint Department of Biomedical Engineering, University of North Carolina, Raleigh, NC, United States

Glutathione is an endogenous antioxidant that forms a component of cellular defences against oxidative stress. Oxidative stress plays a key role in the progression of liver disease. We postulated that in vivo measurements of glutathione synthesis rate and concentration could provide a non-invasive biomarker of hepatic oxidative stress defences sensitive to the progression of liver disease. We employed MR spectroscopy to measure the metabolic incorporation of infused 13C-labelled glycine into glutathione into acute and chronic models of liver oxidative stress. Concentration of labelled glutathione was higher in the acute model, and lower in chronic models, compared to controls.

Abstract PDF Presentation Video

Keywords

acquisition activation acute administration allows altered animals antioxidant arises audience ballooned biomedical biopsy blood carbohydrate cells cellular central chronic coil collagen commenced common comprised compromised concentration content control council dead decoupled decrease decreased demonstrating deposition detect diet disease divided drinking dying dynamic elevated elevation emergence endogenous enrichment environment enzymes every excess experiment experimental extracts fractional function glycine hallmarks healthy hepatic highlights histological histology hours house human in vivo incorporation induce infusion insult insults intracellular invasive kcal kingdom known label larger liver localizer maintenance male many mass measure mechanisms medical medicine metabolic minutes model models monitor monitoring north occurs olive oxidative oxygen pathway peak phantoms physiologically placed potential principal prior processes quantification rats reactive reduced reflect reflecting relevant report required response role samples sections serve species spectra spectral spectrometer spectrometry spectroscopy steady strategy stress studies successfully sucrose supplemented swollen synthesis taken target therapeutic though tissue translated treated treatment upon validated visible water wear weeks