Meeting Banner
Abstract #0536

Metabolite T1 Relaxation Enhancement by Spectrally-Selective Excitation

Noam Shemesh1, Jean-Nicolas Dumez1, Lucio Frydman1

1Department of Chemical Physics, Faculty of Chemistry, Weizmann Institute of Science, Rehovot, Israel

Modification of proton T1 relaxation upon band-selective excitation is well known in protein solution NMR; however, such effects have never been observed in CNS metabolites. Here, we show statistically significant T1 relaxation enhancement in non-water-suppressed band-selective excitation for several metabolites in excised mouse brains. A 30-50% decrease in T1 was observed compared to conventional water suppressed schemes, suggesting some form of cross-relaxation as a mechanism. Our methodology affords a straightforward way to enhance MRSs sensitivity per unit time, and opens new routes to investigate the nature of metabolic interactions among them and with water in tissues, at a molecular level.

Abstract PDF Presentation Video

Keywords

able abundant affect affording although another arise attributed backbone band bands biological brain brains buildup chem clearly contrast contribute counterpart counterparts covering cross crusher curve curves decay designed despite detect detected deterioration directly discovered distortions dong effective efficiency enhance enhancement entirely established even exceptional exchange exchanging excised excitation excited expected extend extended faster fields findings fine fitting fresh governed hence hypothesized importantly improved in vivo indeed integrity intensity inversion jean knowledge lattice lead longer magnet magnitude measure mechanisms metabolic metabolite metabolites micro modifications modulation molecular mostly mouse needed noise note notice occurs offer open optimal partner peaks phenomenon practical properties property protein protons pulse pulses quantification refocusing remarkable repetition requiring reservoir respectively saturation scheme science seems selective sensitivity shorter since slight solution specimens spectra spectral spectrally spectroscopy spectrum spin statistical statistically straightforward structural structure studied subsequently suggesting suppressed suppression systems taken tissue tissues tube unassigned understanding unit upfield upon valuable varying water whatsoever