Meeting Banner
Abstract #0449

3D MRI Microscopy of the Lens, Vitreous, and Anterior Chamber Volumes in Normal Mouse Eyes and Eyes with Retinal Degeneration

Eric R. Muir1, Timothy O. Duong2

1Research Imaging Institute, University of Texas Health Science Center, San Antonio, TX, United States; 2Research Imaging Institute, UT Health Science Center at San Antonio, San Antonio, TX, United States

Retinitis pigmentosa (RP), which causes photoreceptor death and blindness and affects 1.5 million people worldwide, is characterized by progressive loss of the retina. While the retinal degeneration is well characterized, it is unclear how other structures of the eye in RP are affected. The goal of this study was to develop a 3D MRI approach to image the whole mouse eye in vivo at 47x78x79 &[micro]m with high contrast and sensitivity using balanced steady state free precession. We applied this approach to quantify volumes of the lens, vitreous, and anterior chamber in a mouse model of RP.

Keywords

additionally affected affecting allows among analyzed anatomical anesthesia anterior artifacts assume audience automatically averaging avoids background balanced becomes begins boundary brackets central chamber characterized coil combined completed conducted contrast control controls cycling days degenerates degeneration destructive detection determine develop dilated diseases distinguished distortion earlier edge encoding eyes females free frequency frequently full goal gradient health highly histological histology humans in vivo included indicate indicating intensive invasive iris isotropic labor larger lens linear longitudinal loss maintained males matrix mice microscopy million misalignment model monitored monitoring mostly mouse needed nerve outlines parallel people post posterior potential precession pupil quantify readily reason relatively remarkable remove rendering representative researchers resolution respiration retina retinal scanner science sectioned sectioning seemed segmentation segmented semi sensitivity sets sexes significance significantly since slice smaller steady structure structures studies summary surfaces symmetry target temperature thinned timothy tissue translated treatments type unaware unclear urethane usually virtual vision vitreous volume volumes whether whole wild worldwide