Meeting Banner
Abstract #0418

Magnetic Resonance Imaging of Neuronal Currents in an in Vitro Turtle Cerebellum

Padmavathi Sundaram1, William Wells2, Darren B. Orbach1, Daniel Orringer3, Mukund Balasubramanian1, Robert Mulkern1, Yoshio Okada4

1Radiology, Children's Hospital Boston, Harvard Medical School, Boston, MA, United States; 2Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, United States; 3Neurosurgery, University of Michigan, Ann Arbor, MI, United States; 4Neurology, Children's Hospital Boston, Harvard Medical School, Boston, MA, United States

This work investigates the relationship between neuronal currents and MR signals in an in vitro bloodless turtle cerebellum (Cb). While prior work has demonstrated sensitivity of MR signals to currents, the spatiotemporal relationship between MR phase and neuronal currents remains to be characterized in brain tissue. We use the turtle Cb because: (1) there are no signal contributions from blood, respiration or motion, (2) the cellular circuit is intact, (3) the preparation can withstand anoxia and is physiologically functional for several hours, and (4) it is a flat tissue with the principal neurons oriented parallel to each other.

Abstract PDF Presentation Video

Keywords

acrylic activity agreement amplifier appear approved approximately arbor artifacts assume averaging axis biological black block blood bloodless blue bottom brain care cerebellar cerebellum chamber children circuit coated coil cold compatible component compute computed concurrently conditions contributions courses creates cutoff decapitation density detection differential dipole distributed duration eared electrode electrodes enable encouraging entire evoked evoking except excitatory field fields filtering flat flow functional generate generators geometry glucose gradient green ground half hemisphere hospital hours ideal institution intact inter investigates linear local magnitude manually match matrix medical motion negative neurology neuronal neurosurgery nylon oriented overlaid oxygen partial pass peak peduncle peduncles phantoms pharmacological placed positive potentials predictions preparation principal prior procedure pulses radiology receive record recorded recording recovery related relationship removal removed response ringer scanner school sensitivity side since slice slow solution source spatial step stimulated stimulating stimulus string studies subcommittee surf surface symmetry tissue trials turtle twisted unlike vitro wash withstand women