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Abstract #0359

Multi-Parameter MRI Analysis of the Time Course of Induced Muscle Damage and Regeneration

Donghoon Lee1, Shu Feng1, Daniel Chen1, Martin Kushmerick1

1University of Washington, Seattle, WA, United States

We analyzed the time course of changes in mouse hind-limb musculature following localized myotoxin injection by T1, T2, magnetization transfer (MT) and diffusion MRI. Diffusivity and edema increased within the first hours after toxin injection, and faster than the rates of rise of both T2 and MT ratio. Volume decreased faster than T2 and ADC during regeneration. MT returned to control the slowest. Histological analyses confirmed the underlying cellular changes. The kinetics of change in the MRI modalities can be distinguished during muscle damage and regeneration and provide a basis for specific MRI methods to distinguish the underlying cellular processes.

Keywords

acute analyses analyzed animal arrow arrows available basement blue bore cell cells cellular characterize clear clinical coil conducted constant constants contrast control controls corp correlate course courses curves damage damages days degeneration detect development diagnostic differentially diffusivity dimensional directly discriminate disorders distinguish edema events evolution expected falling feasibility features fitted focal fourteen fragmentation frank frequently full function gradient green histological histology horizontal hypotheses identifying induce induced inflammation initial injected injection inner intensity kinetics knee limb localized macrophages made magnet magnetization male matched maximal measure measured membranes mentioned mice microscopic modalities modality model mouse muscle muscles musculature necrosis noninvasive noted noticed occurring occurs parallel parametric pathological physiological post practice processes progression pulse rapid recovery regenerating regeneration regression relevant representative rise rising satellite scanner scientific secondly sections separation several skeletal slice spares spectrometer staining statistically structural structure studied subsequent temporal temporally thereafter tissue tissues toxin transfer underlying volume weeks whether white wide yellow