Meeting Banner
Abstract #0355

Skeletal Muscle State Estimation by T2 and Rotating Frame Relaxations in Ischemic Hind Limb Mouse Model

Hanne Hakkarainen1, Galina Dragneva, Petra Korpisalo-Pirinen, Seppo Yl-Herttuala, Olli Grhn, Timo Liimatainen

1 A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland

T2 and rotating frame relaxation time constants were applied to estimate ischemic muscle viability in a mouse hind limb ischemia model with and without AdhVEGF-D gene therapy. On ischemic leg, percentages of pixels where relaxation times were at the same range as in intact leg were determined and correlated with normal areas determined from histology. High correlations were found with the relaxation times studied (T2, T1&[rho], TRAFF2), indicating that they could be used to evaluate ischemic muscle state non-invasively and provide potential imaging marker for therapy outcome.

Abstract PDF Presentation Video

Keywords

academy adiabatic altering anesthetized anisotropy applications applied artery assessment assumed better bone brain coil consisted constants control correlated correlation correlations created cross days demand determined differentiated diffusion disease divided double drawn early eastern effective eight either especially estimation evaluate excluding fast female femoral fictitious field foundation fraction frame gene green half hand hard highest hind histological histology increasing indicated injection institute invasive last lead length lengths ligated ligation limb lock made maps marked marker markers materials matrix mediated medicine mice model molecular mouse muscle muscles myocardial necrotic operation outcome percentage personalized pixels potential preparation prepared progression pulse pulses quantitative radiology readout receiver recently recovery regenerating regeneration regenerative representing represents rest rotating sacrificed saturation sciences section sections several short significantly skeletal skin slice spin staining subcutaneous suggest suppression surface therapy thresholds tissue tissues towards train transmitter treated trend tuning vein viability volume water whole