Meeting Banner
Abstract #0299

Neurometabolic and Neurovascular Couplings Across Cortical Layers of Rat Brain

Peter Herman1, Basavaraju G. Sanganahalli1, Douglas L. Rothman1, 2, Hal Blumenfeld3, 4, Fahmeed Hyder1, 2

1Department of Diagnostic Radiology, Yale University, New Haven, CT, United States; 2Biomedical Engineering, Yale University, New Haven, CT, United States; 3Neurology, Yale University, New Haven, CT, United States; 4Neurobiology, Yale University, New Haven, CT, United States

CMRO2 changes were calculated with calibrated fMRI at 11.7T measurements of CBF, CBV, and BOLD responses across layers of the rat cortex. LFP and MUA measurements were compared to BOLD, CBV, CBF, and CMRO2 responses. Surprisingly BOLD and CBV responses varied considerably across layers and were uncoupled with layer-specific neural measures - LFP was strong in all cortical layers, whereas MUA was weakest in the superficial layers. CBF was strongly correlated with LFP, whereas CMRO2 was correlated with MUA. While these results lend experimental support for neurometabolic and neurovascular couplings across the cortex, CBF/LFP and CMRO2 /MUA have different spatial distributions.

Abstract PDF Presentation Video

Keywords

accurate activities activity additionally allowed anesthesia anesthetized animal anterior arterial artificially bandpass beat better biomedical blood bold bore brain burr calculations calibrated cerebral coil confirm considerably considered consumption converter correlated correlation cortex cortical coupling couplings derived describe description diagnostic distributions dose drilled duration electrical electronic engineering equally equation evoked experiment extracellular femoral field filter free function functional functions gases glover good gradient haven horizontal identify identifying impedance indeed independently initial inserted inside iterative laser layer layers literature local measured mere metabolic micro middle minute misleading modal modalities modeled modified monitoring nature neural neurobiology neurology neurons observations origin origins oxford pattern peter phys physiological placed potentials predicted pressure probes process property radiology rats recorded related respectively responses since smith software spatial specificity spectrometer spike stability stimulations strong strongly studies superficial supported surface surgery switched table throughout tiny together trans transfer tungsten uncoupled understanding unit upper variate varied ventilated vibration weakest whereas wiener