Meeting Banner
Abstract #0031

Arterial Spin Labeling for Quantification and Monitoring of Renal Blood Flow Changes After Acute Kidney Injury in Mice Comparison with Histopathology and Renal Function

Katja Hueper1, Marcel Gutberlet1, Dagmar Hartung1, Song Rong2, Hermann Haller2, Martin Meier3, Frank Wacker1, Faikah Gueler2

1Radiology, Hannover Medical School, Hannover, Germany; 2Nephrology, Hannover Medical School, Hannover, Germany; 3Institute of Animal Science, Hannover Medical School, Hannover, Germany

We investigated whether arterial spin labeling (ASL) allows monitoring renal perfusion impairment after acute kidney injury (AKI) in mice. Moderate and severe AKI were induced and renal blood flow (RBF) was measured by ASL using a 7T scanner. At day 7, RBF was significantly reduced after moderate and severe AKI. RBF returned to baseline at d28 after moderate, whereas it remained significantly reduced after severe AKI. RBF-changes correlated with impairment of renal function, renal fibrosis and kidney volume loss. Thus, ASL may help to non-invasively detect renal perfusion impairment and presence and severity of AKI at an early time point.

Abstract PDF Presentation Video

Keywords

acute allows alterations alternating anesthetized animal animals arterial artifacts assessed audience axial black blood capillaries chronic clamping clearance compensated coronal correction correlate correlated cortical damage days decrease determined early evaluated example examples experimental fair fibrosis filtration flow frank fully function functional furthermore greater green haller histological histology impairment indicate indicating induced inflammation injury institute invasive inversion investigate kidney kidneys labeling leads loss manual maps marked martin matrix measured medical mice minimal model models moderate monitoring morphological motion mouse note percentage perfusion physicist predictive presence prior progressive pronounced quantification quantified radiologists radiology recovery reduced reduction registration remained renal respiratory returned rigid saturated scanner school science segmentation sensitive severe severities severity significantly similarly slice song spin staining studies surgery target till tissue transient triggered turbo unilateral upper versus volume volumes weeks whether width window