Meeting Banner
Abstract #4137

Optimization of QBOLD Methods for the Assessment of Mouse Renal Oxygenation

Feng Wang1, 2, David A. Hormuth, II1, 3, Keiko Takahashi4, John C. Gore1, 2, Raymond C. Harris4, Takamune Takahashi4, Christopher C. Quarles1, 2

1Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States; 2Radiology and Radiological Sciences, Vanderbilt University, Nashville, TN, United States; 3Biomedical Engineering, Vanderbilt University, Nashville, TN, United States; 4O'Brien Mouse Kidney Physiology and Disease Center, Vanderbilt University, Nashville, TN, United States

Renal function is highly dependent upon adequate perfusion and oxygenation and their non-invasive assessment in mice could provide useful tools with which to interrogate disease models. Recently, an experimentally practical quantitative BOLD approach (qBOLD) was proposed that enables the quantification of local blood oxygen saturation (LSO2) [1]. In this study, we evaluated the feasibility of this qBOLD protocol for application to mouse kidneys. Specifically, we optimized imaging sequences and protocols that enable mapping of T2, T2* (before and after the injection of a contrast agent), blood volume fraction (BVf) and Bo.

Abstract PDF Presentation Video

Keywords

abnormal accumulations acquisition adequate agent anesthetized application applied arrows arteries artifacts assay assessment biomedical biophysics blood body bold caliber challenges characteristic christen coil compartments compatible comply computed considered contrast correction cortex created currently decay dependent described detect disease dosage dosages doses echoes enable enables engineering evaluated example exhibited experimentally feasibility fitting flow fraction frame function gating general good gore gradient head highly homogeneous improve indicate influence initial injection inner insights institute interrogate invasive investigating iron john kidney kidneys likely local maintained manipulation mapping maps matrix measured medulla mice minimize minutes model models monitored motion mouse optimization optimized outer oxide oxygen oxygenation papilla parametric partial particularly pharmacological physiology potentially practical preliminary previously proposed protocol protocols quantification quantitative radiological radiology rapid reflecting relatively reliably renal required resolution respiration saturation schemes science sciences session shed slice slightly spacing specifically stabilized strip system temperature throughout tools upon useful validate validated veins volume ways whether white