Meeting Banner
Abstract #3908

Non-Invasive Identification of Functional Brown Adipose Tissue in Rodents Using Hyperpolarized 13C Imaging

Angus Z. Lau1, 2, Albert P. Chen3, Michelle Ladouceur-Wodzak1, Krishna S. Nayak4, Charles H. Cunningham1, 2

1Imaging Research, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada; 2Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada; 3GE Healthcare, Toronto, Ontario, Canada; 4Department of Electrical Engineering, University of Southern California, Los Angeles, CA, United States

The recent identification of functional brown adipose tissue (BAT) in humans has implications for the treatment of obesity. In this study, dynamic, spatially resolved hyperpolarized 13C imaging was used to non-invasively identify functional BAT in vivo in a rodent model. BAT was activated using intraperitoneal injection of norepinephrine and detected following infusion of pre-polarized 13C pyruvate. Increases in 13C bicarbonate (3.7-fold) and 13C lactate (3.5-fold) signal in the interscapular region correspond to a known rodent BAT deposit. The radiation-free nature of this imaging exam may potentially facilitate trials of therapeutics targeting BAT activation in humans.

Abstract PDF Presentation Video

Keywords

acids activated activation additional adipose adult aerobic agents alternative anaerobic anesthetized animal animals arrows axial bicarbonate biophysics blood brown capacity cardiac carries clinic coil complete conditions consistent consumption contributing corrected courses cropped currently dashed deposit deposits depot developed development diseases dorsal dose draining drawn electrical engineering especially facilitate failure fatty feasibility flux fold free functional future health healthy heart hour humans ideal identification identify implications in vivo independent indicate indicates infusion injection invasive juveniles known lactate located male mediated medical metabolic metabolism metabolite metabolites minute minutes modality model nature nelson nominal normalized novel obesity otherwise oxidative oxygen polarized population position potential potentially prepare presumably previously primary protein pulse radiation rats recent representative requires resolution resolved risk rodent rodents room sample scanned scanner sciences separated shivering shot side simultaneously slices solid southern started stimulated stimulation stroke studies substrate supine targeting temperature temporal therapeutics tissue translation treatment trials uncoupling undesired upon venous volume water whether