Meeting Banner
Abstract #3867

Glioma Cells Transfected with Mms6 Enhance Cellular Iron Oxide Nanoparticles Uptake and Retention in Vitro

Xiaoyong Zhang1, Brenda Robledo1, Jie Song1, Xiaoping P. Hu1

1Department of Biomedical Engineering, Emory University, Atlanta, GA, United States

Iron oxide nanoparticles (IONPs) and MR reporter genes are tools used for cell labeling and tracking using MRI. In this work, we hypothesized that expressing mms6 gene with IONPs could enhance cellular IONPs uptake and retention. After culturing with IONPs, compared to mms6-negative tumor cells, mms6-positive tumor cells contained a higher level of intracellular iron and exhibited a higher R2. In addition, mms6-positive cells retained more iron with cell passages. These results indicate that expression of mms6 can potentially enhance cellular uptake and retention of IONPs. This capability of mms6 may allow us to develop a mean for targeted uptake and retention of IONPs for MR molecular imaging.

Keywords

absorption accumulating alliance allow annual bacteria bars bind biomedical blot blue capability cell cells cellular chem clone coated collected color concentration concentrations confirmed consistent contained content contrast core counted cultured dark described determine develop diluted division echoes endogenous engineering enhance error examined expressed expressing expression fixed gene generate genes gravity growth hand histology hour hours hypothesized increasing incubated incubation indicate indicates indicating induced insufficient intracellular inverted iron known labeled labeling limited lost materials measure measured media medical meeting metabolized microscope molecular ocean oral oxide parental passage passaged passages pellets positive potential potentially previously proceeding produce quantified rapidly read reader remaining reporter respectively retention robustness room scanner selected settling significantly solutions song stained staining statistically strong studies successively supplemented supported targeted temperature tools track tracking transverse tube uptake various vitro wavelength western whether whole widespread