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Abstract #3681

MRI of Acute and Delayed Administration of Marrow Stromal Cells in Rats with Traumatic Brain Injury

Lian Li1, Michael Chopp1, 2, Guangliang Ding1, Changsheng Qu3, Qingjiang Li1, Siamak P. Nejad-Davarani1, Quan Jiang1

1Neurology, Henry Ford Hospital, Detroit, MI, United States; 2Physics, Oakland University, Rochester, MI, United States; 3Neurosurgery, Henry Ford Hospital, Detroit, MI, United States

While cell transplantation has potential as an effective therapeutic strategy to attenuate secondary injury after traumatic brain injury (TBI), several key issues regarding optimization of basic transplantation techniques, such as timing of transplantation, remain to be addressed. Using MRI, we tested the hypothesis that acute engraftment of human marrow stromal cells (hMSCs) into the brain subjected to TBI provides an advanced therapeutic effect as compared to delayed transplantation. Our data demonstrate that acute cell intervention extends the time range of therapeutic benefit by initiating the therapeutic effects earlier, resulting in an enhanced protective and therapeutic effect.

Keywords

acute administration advanced affects agent animals atrophy attenuate background barrier basic benefit blood bone brain cell cells central cerebral confirmed constant contrast controlled cortical days decrease delayed derived detected diffusely dilation dimensional diminished ding distinct distinguished earlier early elevated enhanced evaluated evokes expansion extends flow ford function functional gradient graphs henry histological hospital hours human hypothesis identify important in vivo indicating induced inflammatory initiating injected injection injured injury intense intervention intravenous intravenously issues lateral lesion likely male maps marrow materials median monitored nervous neurological neurology neurosurgery objective observation occurred occurs optimization outcome outcomes period permeability physics plays post presence promising protective quantified rats reduce reduced reduces reduction regarding remain remodeling repair respectively response restores role saline score secondary several severity side significantly slice spatial stained strategy stroke subjected suggest suggesting suspension system temporal therapeutic therapy timing tissue tracked transfer transplantation trauma traumatic treated treatment ventricle ventricles ventricular vessels volume volumetric weekly wide window