Meeting Banner
Abstract #3675

Multimodal MR Evaluation of Experimentally Induced Apoptotic Neuronal Death in the Rat Brain

Yohan van de Looij1, 2, Volodomyr Petrenko3, Petra Susan Hppi1, Rolf Gruetter4, 5, Jozsef Kiss3, Stphane V. Sizonenko1

1Division of Child Growth & Development, University of Geneva, Geneva, Switzerland; 2Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland; 3Department of Fundamental Neurosciences, University of Geneva, Geneva, Switzerland; 4Laboratory for Functional and Metabolic Imaging, cole Polytechnique Fdrale de Lausanne, Lausanne, Switzerland; 5Department of radiology, University of Geneva and Lausanne, Geneva and Lausanne, Switzerland

Despite the importance of apoptosis in the brain pathology, in vivo diagnostic of this process is still not developed. The aim of this work was to evaluate the possibility of using non-invasive MR techniques to detect apoptotic neuronal death in the rodent cerebral cortex. Neuronal ablation was induced by using a diphtheria toxin and diphtheria toxin receptor system. MRS and DTI detected metabolic and micro-structural alterations induced by apoptotically dying neurons within the cortex. Further experiments are in progress to assess precisely acute and long-term apoptosis-induced MRI changes.

Keywords

absolute achieved acquisition activated activation actively activity adiabatic antibodies assess atlas became body brain brains capsule cause cell cells cerebral coils concentration confers confocal consistent constructs corrected cortex cortical cortices crosstalk days death decrease degeneration delineated despite detect detected developed development diagnostic diffusion diffusivity diphtheria disruption double dying eddy eliminated equal evaluate extent external five fluorescent fond foundation functional functions genetically gradient green histological immediately in vivo indicated indicating induce induced injection integrity invasive kiss known labeled layer leading lesion lesioned loss magnet manually maps marker metabolites model morphological natal necrosis neural neuronal neurons nuclear organelle overall overlaid parametric pathologies place population populations position positive principally processes profile programmed protein protocol providing purple radiology rats reached reactions reactive receptor residual rodent shielded shimming short shrinkage side significantly site spectra spectral spectroscopy stained still striking stroke structure subjected suffering summed surface system targeted tendency tensor tissue together toxin typical ultra unilaterally utilizing volume water