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Abstract #3430

NMR Metabolomics of Drug Response to Antineoplastic Polyherbal Formulations Studied in Human Hepatocellular Carcinoma Cells

G. Sharma1, Rama Jayasundar1, T. Velpandian2, R. Singh3, S. S. Chauhan3, V. Kapoor4, S. N. Das4

1NMR, All India Institute of Medical Sciences, New Delhi, India; 2Ocular Pharmacology & Pharmacy, All India Institute of Medical Sciences, New Delhi, India; 3Biochemistry, All India Institute of Medical Sciences, New Delhi, India; 4Biotechnology, All India Institute of Medical Sciences, New Delhi, India

The study has used NMR metabolomics to profile drug response of Hep-G2 tumor cells to treatment by chemotherapeutic drug paclitaxel and four polyherbal formulations, all of which had antineoplastic activity assessed by apoptosis detection assay. Proton spectrum of untreated cells showed prominent resonances between 3.5-3.8ppm. These peaks showed drastic reduction in response to treatment by paclitaxel and formulations. In addition, there were also other spectral changes. PCA analysis of the spectral data of drug induced metabolic changes showed a clear difference between the untreated and treated cells with the spectral response to paclitaxel and one of the formulation being similar.

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Keywords

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