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Abstract #1986

Redox Map of Mouse Brain by Three-Dimensional EPR Imaging with Six-Membered Nitroxyl Radicals

Miho C. Emoto1, Hideo Sato-Akaba2, Hiroshi Hirata3, Hirotada G. Fujii1

1Center for Medical Education, Sapporo Medical University, Sapporo, Hokkaido, Japan; 2Department of Systems Innovation, Osaka Universivy, Toyonaka, Osaka, Japan; 3Division of Bioengineering and Bioinformatics, Hokkaido University, Sapporo, Hokkaido, Japan

Six-membered nitroxides such as TEMPOL and TEMPONE are redox-sensitive probes for redox research, but due to their short lifetime in vivo, they have not been fully examined in EPR imaging studies in living animals. In this study, an improved EPR imaging system with a rapid-field scanning capability was used, and three-dimensional EPR images of mouse heads were obtained after administration of TEMPOL and TEMPONE. Half-life maps of TEMPOL and TEMPONE in mouse heads were calculated from a series of EPR images recorded at an interval of 16 s. Obtained half-life maps clearly indicate the redox status of the mouse brain.

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