Meeting Banner
Abstract #1858

in vivo Imaging T Cells in the Rheumatoid Arthritis with Nano-Sized Iron Oxide Particles by MRI

Chih-Lung Chen1, Cheng-Hung Chou2, Chen-Hsuan Lin1, Wen-Yuan Hsieh1, Shian-Jy Wang1, Chen Chang2

1Industrial Technology Research Institute, Hsinchu, Taiwan; 2Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan

The activation of T-cells correlates with the pathogenesis of rheumatoid arthritis (RA). Understanding the genesis, migration, and distribution of the T-cells provides important perspectives regarding RA. We developed an optimal carboxylated group of iron-oxide particles and adapted it to conjugate with CD3 antibody. We then investigated the labeling efficiency and binding specificity of the nano-sized iron oxide-based contrast agent IOPC and IOPC conjugated CD3 by cellular MRI via intravenous infusion. Specifically, we have detected a migration and accumulation of the T-cells in the arthritis joint in a rat RA model with in vivo MRI by IOPC-CD3.

Abstract PDF Poster PDF

Keywords

abnormality academia according accumulation activation administration adsorption agent allowed anatomy antibodies arthritis assessment biomedical blood bloodstream blue bone booster carried carry cartilage cell cells cellular circulation coated coil collagen condition contrast contribute controls correlate days decrease decreased detect detection developed development diseases distribution duration fast female femoral field glycol growth harvested help hung immunization important in vivo incomplete induced infiltration inflammatory infused infusing initiation injected injection inner institute interventions intravenously involved iron joint knee knees label length lesioned lewis liver located longer lung major marrow massive materials matrix mature medicine merged migration minimal model monitored monitoring noise nonspecific observation onset onto particle particles pathology perspectives plate polyethylene population post powerful presumably proteins quadrature quantification rats received receiver reducing regenerative repeatedly reveal revealed rheumatoid saline sciences section selection sham simulate site slice spectrometer staining strong studies suggested surface targeting therapeutic tissue train transmitter transverse type typically understanding uptake verified verify vessels volume widely yuan