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Abstract #1852

Mammalian Expression of a CEST Reporter Gene Based on Human Protamine-1

Amnon Bar-Shir1, Guanshu Liu2, Kannie W.Y. Chan2, Michael T. McMahon2, Peter C.M. van Zijl2, Jeff W.M. Bulte1, 3, Assaf A. Gilad1, 4

1Radiology, Johns Hopkins University, Baltimore, MD, United States; 2Kennedy Krieger Research Institute, Baltimore, MD, United States; 3Institute for Cell Engineering, Johns Hopkins University School of Medicine,, Baltimore, MD, United States; 4Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, United States

We have developed a novel CEST-MRI reporter gene based on human protamine-1, and demonstrated its expression and detection in mammalian cells, as well as in a three-dimensional cell culture model of immune-isolated cells. Due to its biocompatibility, this humanized reporter gene is expected to have higher cellular tolerance with minimal immune response.

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