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Abstract #1660

Bio-Integration Assessment of Rat Knee Cartilage Repair Using in vivo MRI at 7T

Olivier Beuf1, Jean-Christophe Goebel2, Emilie Roeder3, Anne-Laure Perrier2, Denis Grenier2, Pierre Gillet3, Astrid Pinzano3

1Universit de Lyon; CREATIS; CNRS UMR 5220; Inserm U1044, Universit de Lyon, Villeurbanne, France; 2Universit de Lyon; CREATIS; CNRS UMR 5220; Inserm U1044, INSA-Lyon; Universit Lyon 1, Villeurbanne, France; 3PPIA, UMR 7561 CNRS Nancy Universit, Vandoeuvre-Ls-Nancy, France

The biointegration of biomaterials colonized by mesenchymal stem cells or not after implantation in a calibrated full thickness focal lesion in the rat knee were followed using 7T HR-MRI. A four-channel surface coil designed for the simultaneous acquisition of both knee joints of a rat was used. The quality of the MR images obtained with the developed array coils allowed the segmentation of cartilage compartments and measurements of cartilage thickness and volumes. HR-MRI allowed the follow up of cartilage to evaluate in vivo therapeutical responses after chondrogenic biomaterial grafting.

Keywords

acceptance according accurate achieve acquisition adult allowed allowing animal animals array articular assessment bandwidth blanc calibrated capability cartilage cartilages cell cells challenging channel channels coil coils collagen colonized committee comparable compartments compute confrontation constant contouring counting course datasets dedicated defect defects derived designed developed dimensional directly disease dislocation done drawn drill ethics evaluate examine experimental experimentation exploration exposed femoral field fixed flash focal framework full functionality funded graft grafting grant groove guidelines handled histological home human implantation in vivo incision integration interactive investigations joint joints knee knees lateral lesion lewis made male mandatory manual material matrix medial nude osteoarthritis outer partitions patella pathologic pixel powerful primes procedure proprietary qualitative quality quantitative rats real receiver repair require required resolution respectively responses rodent sacrificed screen script seeded segmentation segmented sets sham side significantly simultaneous slices software sponges stem strictly studies surface surgical system therapies thick throughout tibial touch transfer transplantation underwent validated visualize volume volumes