Meeting Banner
Abstract #1584

in vivo Imaging of Free Radicals in Cardiac Tissues from Diabetic Mice with the Use of MRI

Rheal A. Towner1, Nataliya Smith1, Debra Saunders1, Jorge Carrizales1, Florea Lupu2, Robert Silasi-Mansat2, Kenneth Humphries3, Shraddha Vadvakar3, Marilyn I. Ehrenshaft4, Ronald P. Mason4

1Advanced Magnetic Resonance Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 2Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 3Free Radical Biology & Medicine, Oklahoma Medical Research Foundation, Oklahoma City, OK, United States; 4Laboratory of Pharmacology & Chemistry, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States

Free radicals have been shown to play a major role in the pathogenesis associated with cardiac problems in diabetes. This study reports on in vivo imaging of membrane bound radicals (MBR) with the use of molecular MRI (mMRI) and immuno-spin trapping (IST) on diabetic cardiac muscle in a streptozotocin (STZ)-induced model. The spin trapping compound DMPO (5,5-dimethyl-pyrroline-N-oxide) is used to trap radicals that can be recognized by an anti-DMPO probe with an albumin-anti-DMPO-antibody-Gd-DTPA-biotin construct. This method can be used to understand the role of free radicals in cardiac-related pathogenesis in diabetes.

Abstract PDF

Keywords

accumulation acquisition acute adducts administered administration agent albumin allowed anti antibodies antibody assessment assignments biology biotin blood bore bound breast cardiac cardiovascular characterized chemistry circulation city coil combined compound consecutive considered construct contrast control controls coronal correspond daily days depicting described detection diabetes diabetic dimethyl disease diseases either elevated elite environmental evaluate every evidence excised exclusively experimental fast field fluorescence foundation free funding general glucose gradient health heart highly horizontal hyper immune in vivo included induced initiated injected injection instead intensities intensity laboratory landsman lipid lungs lymphatic mason matrix measured medical medicine mice microscopy moderate moiety molecular mouse muscle nonspecific note observe opportunity oxidative oxide percent placed post presence previously prior probe protein protocol quantitative radical radicals repetition reports role rung saline sciences severe shot slices smith spectrometer spin started steps stress stresses strip studies suggests supporting synthesis tail taken targeted thresholded tissue tissues towner trapping treatment triangle uptake vascular ventricular view weeks