Meeting Banner
Abstract #1364

An Efficient Protocol for Infarct Quantification in Mice

Guido Buonincontri1, Carmen Methner2, Thomas Krieg2, T. Adrian Carpenter1, Stephen J. Sawiak1, 3

1Wolfson Brain Imaging Centre, University of Cambridge, Cambridge, United Kingdom; 2Department of Medicine, University of Cambridge, Cambridge, United Kingdom; 3Behavioural and Clinical Neuroscience Institute, University of Cambridge, Cambridge, United Kingdom

Late gadolinium enhancement is a remarkably accurate way of assessing tissue viability after myocardial infarction. Performing this technique in mice is useful for translational drug studies carried out longitudinally in order to monitor adverse remodeling. In mice, high cardiac rate and small size mean that standard TI-optimised inversion recovery is not as efficient as in humans. To achieve LGE imaging efficiently we propose and validate a multi-slice method with an optimised acquisition scheme. Our method achieves excellent hyperenhancement and CNR and does not require TI optimization.

Keywords

achieved acquisition acquisitions administration adverse agent alternate apex approaches artifacts assess assessment audience axis best better bias bland brain building carpenter chloride chosen cine clinical cohort comparing confirmed containing context contrast correlation covering delay delineated described diastole directed drug duration easy efficient elevation employed enhancement enhancements established every excellent experiment fast fixed gadolinium gated good greater healthy heart heartbeat histology humans identified immediately implement important in vivo induced infarct infarction infarcts injection instance intercept interleaving intervals inversion kingdom late least location locations long maintained male manually marked matrix measured medicine mice minute minutes models mortality motion mouse must myocardial observe optimal optimization overcome pathological percentage phantom phys plot potential preparation price problems procedure protocol protocols pulses quantification radiology recent recovery reduces relies remote require respiration respiratory revealed rodents sacrificed scheme segmented select shape short slice slices slope stained staining still strategy sufficient target thick tissue trigger twenty varying ventricle viability vials water whole