Meeting Banner
Abstract #0616

Parametric MRI Reveals Vascular Effects of Antibodies to the &[alpha]1-Adrenergic Receptor by Demonstrating a Reduction in Relative Cerebral Blood Volume (RCBV)

Andreas Pohlmann1, Babette Dieringer1, Peter Karczewski2, Natali Wisbrun3, Irina Palatnik1, Christina Eichhorn4, Petra Hempel2, Rudolf Kunze5, Marion Bimmler6, Thoralf Niendorf1, 7

1Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck Center for Molecular Medicine, Berlin, Germany; 2E.R.D.E.-AAK-Diagnostik GmbH, Berlin, Germany; 3Animal Facilities, Max Delbrck Center for Molecular Medicine, Berlin, Germany; 4IT Department, Max-Delbrueck Center for Molecular Medicine, Berlin, Germany; 5E.R.D.E. e.V., Berlin, Germany; 6Autoimmunity and G Protein-Coupled Receptors, Max Delbrck Center for Molecular Medicine, Berlin, Germany; 7Experimental and Clinical Research Center, a cooperation between the Charit Medical Faculty and the Max Delbrck Center for Molecular Medicine, Berlin, Germany

Evidence suggests Alzheimers Disease (AD) may be primarily a vascular disease. Recently, agonistic autoantibodies to the &[alpha]1-adrenergic receptor (&[alpha]1-AR) were found to cause impairments of blood flow in larger vessels (TOF-MRA) in the rat brain. This work examines the long-term effects of α1-AR antibodies on relative cerebral blood volume (rCBV) in rats. Estimation of rCBV by ΔR2*/ΔR2 mapping in conjunction with an intravascular contrast agent demonstrated a significant reduction in rCBV for &[alpha]1-AR antibody treatment. This data underpins the pathogenic significance of autoimmunity to the &[alpha]1-AR for AD and vascular dementia.

Abstract PDF Presentation Video

Keywords

accompanied administered agent aimed albumin allocated analyses anesthesia angiography animal animals antibodies antibody aorta applied autoimmunity blood body bottom bovine brain cause caused central cerebral cerebrum clinical combined community conceivably conducted confirms conjunction consistent contrast control cooperation coronal coupled covered decrease dementia developed development diabetes disease diseases division dorsal employing entire established evidence experimental facility fact field filter flight flow gradient grant half histogram histograms histological hypertension immunized impairments importance included indicates induced inferences injected injection injections involvements larger largest long macroscopic male mapping maps marked materials matrix medicine microscopic minutes model molecular monthly months much nearby notwithstanding observations parametric pathogenic peptide potential power previous program pronounced protein protocol quantitative random rats received receptor receptors reduced reduction reflected related relatively repeated respective scale segmentation sensitivity serum since smaller still stroke subtraction suggested suggests surface susceptibility system theory third towards treatment type types ultrahigh underpins underscored vascular ventral vessels volume weeks widespread