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Abstract #0263

Myocardial ATP Turnover Rates in the in vivo Hearts with Post-Infarction Remodeling

Qiang Xiong1, Pengyuan Zhang1, Lei Ye1, Jinfeng Tian1, Cory Swingen1, Albert Jang1, J. Thomas Vanghan1, Jianyi Zhang1

1Center for Magnetic Resonance Research, University of Minnesota, Minneapolis, MN, United States

A novel indirect magnetization saturation transfer (MST) approach is developed to allow measurement of ATP→Pi reaction rate without quantification of inorganic phosphate (Pi) levels, thus avoided the primary barrier for applications in in vivo heart. With the new approach, myocardial ATP→Pi reaction rate was measured in Normal and post-infarct hearts with or without stem cell therapy. The data suggested that the ATP→Pi rate is a sensitive bioenergetic index that is tightly correlated to cardiac workload as well as the severity of post-infarction left ventricular remodeling based on a swine model of post-infarction LV remodeling.

Keywords

abnormality accurate altered among animals applications applied approaches avoids barrier better biochemistry border bore capacity cardiac cardiovascular catecholamine cell cells compensated conditions console constant contrast convention conventionally correlated correlation developed difficulties diseased double experienced experiment flux frequency fully functional heart hearts heterogeneous hydrolysis identical immediately in vivo included index indirect indirectly induced infarct infarction infusion injured injury inorganic interfaced kinetics limit linear linearly lost magnet magnetization measurable measure measured measures metabolism million model muscle myocardial myocardium newly outcome peaks phosphate physiology pigs plots post pressure primary prior product production quantification questions reaction reduction regulate regulated related relation relaxed remodeling remote require response revealed saturation sensitive severe severity significantly skeletal stand stem subtracting suggests summary supported swine synthesis therapy tightly transfer transplanted treated turnover unsettled utilized validated ventricular weeks widely workload zone