Meeting Banner
Abstract #0083

Microscopic 3D-DTI of Tumor Cell Migration, Numerical Modeling and Two- Photon Microscopic Imaging

Ulysse Gimenez1, Florence Appaix1, Teodora-Adriana Perles-Barbacaru1, Franck Mauconduit1, Marie-France Nissou1, Emilie Langard1, Laurent Pelletier1, Francois Berger1, Didier Wion1, Boudewijn van der Sanden1, Hana Lahrech1

1Grenoble Institute of Neurosciences, La Tronche, France

A microscopic 3D-DTI is applied on a mouse glioma model using GFP transfected Glio6 cells to detect tumor cell migration. Two-photon microscopy is used for validation. Monte-Carlo simulations of water diffusion in numerical models of cerebral tissue geometry with micro-architecture changes are developed. FA decrease is observed in the corpus callosum where tumor cell invasion is particularly detected by microscopy. Typical elongated tumor cells indicate migration along the fibers. FA changes simulations versus extra / intracellular and white-matter / grey-matter show similar tendency as detected experimentally. The Glio6 model and 3D-DTI constitute a powerful tool to study tumor cell migration.

Keywords

acquisition aims allow analyses analyzed animal applied architecture bearing berger brain brains buffered cancer cell cells cerebral chosen clinically compartment complex composed composite conditions confirmed constitute containing coronal corpus correlation cylinders days decrease density derived detected detecting developed diffusion diffusivity distribution elevated elongated encountered enlargement establish every experimentally extra extracellular fibers fixed fluorescent fractional fractions function geometry gradients green growth histograms hours immersion implantation in vivo incubated indicate individual injected interpreted intracellular invasion invasiveness laser leading letters longitudinal maps marked mice micro microscope microscopic microscopy migration mixture model modeling models monitored mosaics mouse neurosurgery noninvasive novel nucleus nude numerical occurring particularly pathologic pattern perpendicular photon pilot population powerful preliminary preserved progress proposed protein quantitative reduce release reproduce resolution respectively saline scanning section sham simulated simulations since slice slices software solution spatial stem subject suggesting tendency thesis thick thickening thicker throughout tissue tissues together trend tumor typical uniformly validate validation water white whole zoom