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Abstract #4415

Autophagy Alters Lipid Metabolism in Human Colon Carcinoma Cells Evaluated Using 1H NMR

Gigin Lin1, Dow-Mu Koh1, Simon P. Robinson1, Martin O. Leach1, Yuen-Li Chung1

1CRUK and EPSRC Cancer Imaging Centre, The Institute of Cancer Research, Sutton, Surrey, United Kingdom

The intracellular storage and utilization of lipids are critical for cancer cells to maintain energy homeostasis, which is shown to relate to autophagy. In this study, we observed a unique change of lipid profile resulting from drug-induced autophagy. The lipid profile of autophagy shares some common changes with apoptosis, such as increased levels of fatty acids, triacylglycerol and sphingomyelin, which differentiates responsive cell lines from the non-responsive group. The increases of unsaturated fatty acids and phosphatidylcholine could further distinguish autophagy from early stage of apoptosis.

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Keywords

absence acid acids added adherent agents aids allows alters association background blots blotting blue calibration cancer carcinoma cell cells cellular chemical class cleaved colon common condensation confirmed confocal control controls cycle decrease deficient degraded detailed differentiate dose double dried droplets drug dual early either electron energy england environments essential exam expression extraction extracts fatty findings flow fold funding fused generously grant health homeostasis hospital hostile human in vivo induced inhibitor institute integrals interestingly intracellular kingdom leach leading ligand like lipid lipids literature maintaining martin mechanisms membrane memorial metabolism metabolite metabolites microscopy minimal mostly nature necrosis nuclear observe organelles pair paired peak peaks play population presence produce profile profiles prolong prominent proteins reaction recent reconstituted recovery related report representative resistant resolution responsive restricted role roles sequestered significantly society spectra stain starvation status still storage stress summary support supported surrey survival system thank therapeutics tool trail treated treatment treatments unique unsaturated utilization vitro western wild