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Abstract #4377

Temporally Flexible Tracking of Neuro-Progenitor Stem Cells Using Golden Angle Incremented Linear Combination Steady-State Free Procession (LCSSFP) Imaging for Artifact Reduction.

H. Douglas Morris1, J. Andrew Derbyshire2

1NIH Mouse Imaging Facility, National Institutes of Health, Bethesda, MD, United States; 2Laboratory for Cardiac Energetics, National Institues of Health, Bethesda, MD, United States

Rapid individual cell tracking of endogenously labeled stem cells is shown using high-field MRI and balanced Steady-State Free Procession sequences. We present a field artifact suppression technique based on the Golden Angle progression for the RF phase cycle as a modification to Linear Combination SSFP (LCSSFP). The use of golden-angle LCSSFP yields a temporally flexible acquisition scheme that can yield continuous progression of resulting images that do not overlap in pulse-band artifacts. This resulting method can yield very high resolution images quickly with few global artifacts while still tracking magnetic particles.

Keywords

acquiring acquisition additional adult analogous animals apparatus appear applied approximately artifact artifacts axial banding bandwidth bangs brain brains buffered cell cells cellular coil combination concentration console cycle cycles describe detailed detection diameter distortions efficiency energetics entire exploited facility field fishers fixed flexible fold formalin formed forward free frequency golden good gradients greater health in vivo increment incremented individual injected injection institutes intact iron label labeled labeling laboratories laboratory linear local long makes many micro micron microscopy minimal minutes morris mouse move national nearly neural offset oxide parsing particle particles passband perfect perfused phenotype position post precession prevent previously procession produce produces profile progenitors progression progressions projected proven pulse quality radial rapid rapidly rats recently reconstruction reduced reduction relies removed representative require required resolution resolved resonant river rodent scaled selected since sliding solution squared steady stem stored successfully summation summed suppressed suppression temporally tissue tool track tracked tracking twice utility variations ventricles visible weeks