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Abstract #4345

Enhanced Cellular Uptake of Aminosilane Coated Superparamagnetic Iron Oxide Nanoparticles in Mammalian Cell Lines

Xiao-Ming Zhu1, Ken Cham-Fai Leung2, Siu-Fung Lee3, Da-Wei Wang3, Feng Zhao1, Josie M. Y. Lai4, Chao Wan4, Christopher H. K. Cheng4, Yi-Xiang Wang1

1Dept Imaging & Interventional Radiology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong; 2Institute of Creativity and Department of Chemistry, The Hong Kong Baptist University, Kowloon Tong, KL, Hong Kong; 3Department of Chemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong; 4School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong

It was shown aminosilane (SiO2-NH2) coated SPIO (SPIO@SiO2-NH2) had higher labeling efficiency for rabbit mesenchymal stem cells (MSCs) than SiO2 coated SPIO (SPIO@SiO2). This study compared the intracellular uptake of SPIO@SiO2-NH2 with SPIO@SiO2, bare SPIO, and dextran coated SPIO (SPIO@dextran) in RAW 264.7, L929, HepG2, PC-3, U-87 MG cells, and mouse MSCs. All these six mammalian cell lines showed the highest cellular uptake for SPIO@SiO2-NH2. For SPIO@SiO2, bare SPIO and SPIO@dextran, there were variations of labeling efficiency among different cell lines, with SPIO@SiO2 tended to rank as the second, and bare SPIO and SPIO@dextran tended to have lower labeling efficiency.

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Keywords

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