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Abstract #4337

Liposomes Loaded with Paramagnetic Mn SOD Mimetic: Characterization, Relaxometry, and MR Imaging After Systemic Administration

Mohammed Salman Shazeeb1, Giancarlo Feula, 12, Alexei Bogdanov1, 3

1Department of Radiology, University of Massachusetts Medical School, Worcester, MA, United States; 2Worcester Polytechnic Institute, Worcester, MA, United States; 3Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, United States

M40403 is a synthetic manganese based superoxide dismutase (SOD) mimetic that has been extensively studied in vivo and found to have a catalytic activity rate which approaches that of the native Mn SOD enzyme. Here we compared the kinetics of liposome encapsulated M40403 and found that in addition to catalyzing superoxide dismutation In Vitro, M40403 also doubled as a paramagnetic contrast agent in vivo, causing enhancement of mouse brain after systemic administration. Thus liposome encapsulated M40403 is an ideal candidate for development as a theranostic compound useful for MR imaging of SOD activity and disease treatment.

Keywords

ability activation activity actual administered administration agent alter although anion assess background biological biology brain buffer catalytic catalyze cellular cerebral characterization characterized chem chemical choroid circulating clearance closely column components composed compounds concentrations considered contained containing contrast cortex cortical created damaging defense delivery described diameter direct disappeared diseased dissolved doubles efficient efficiently electron eluted enable encapsulation enhancement entry enzyme established exhibit extruding film filtration final fluid formed free function furthermore generate gradient greater health healthy highly horseradish host hydrated immune in vivo incorporated indicate indicated indicating initial injected injection institute invasion lipid lipids loaded long matrix measuring medical membrane mice microorganisms microscope milligram mimetic minutes monitoring mouse native negative observe outward oxygen paramagnetic percent perfusion peroxidase peroxide plates plexus pore post potential potentially preparation produced properties proton quantifications radiology reaction reflecting represented response rotary scattering schematic school shortened since system systemic tail thin transient transition transmission treatment truly uptake useful vascular water yield