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Abstract #4327

In Vivo Enzymatic Assay of Carnitine Acetyl Transferase and AcetylCoA Synthetase Using Hyperpolarized Acetate

Jessica A M Bastiaansen1, Tian Cheng1, Mor Mishkovsky1, 2, Arnaud Comment3, Rolf Gruetter1, 4

1Laboratory of Functional and Metabolic Imaging, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland; 2Department of Radiology, Universite de Lausanne, Lausanne, Switzerland; 3Institute of Physics of Biological Systems, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland; 4Department of Radiology, Universite de Lausanne and Geneva, Lausanne and Geneva, Switzerland

Hyperpolarized MR allows for the characterization of fast biochemical reactions in vivo. Here we studied the metabolic conversion of acetate to acetylcarnitine, a buffer for excess acetylCoA and transporter of acetyl groups. A two-compartment model was used to determine the kinetic rate constants for several substrate concentrations which allowed for the determination of Michaelis-Menten kinetic parameters related to acetate transport and metabolism.

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Keywords

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