Meeting Banner
Abstract #4304

Quantitative Measurement of Pyruvate-Lactate Exchange Rate in Both Directions Using Exchange-Linked Dissolution Agents (ELDA) in Hyperpolarized 13C Metabolic Imaging

Ralph Hurd1, Daniel Spielman2, Sonal Josan3, Jae Mo Park4, Yi-Fen Yen1, Adolf Pfefferbaum3, 5, Dirk Mayer, 23

1GE Healthcare, Menlo Park, CA, United States; 2Radiology, Stanford University, Stanford, CA, United States; 3Neuroscience Program, SRI International, Menlo Park, CA, United States; 4Electrical Engineering, Stanford University, Stanford, CA, United States; 5Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, United States

In hyperpolarized [1-13C]pyruvate and hyperpolarized [1-13C]lactate metabolic imaging, the pool size of the exchange partner may limit the rate of isotopic mixing. This is especially true for hyperpolarized [1-13C]lactate metabolic imaging, given the small steady-state pyruvate pool. Under conditions where addition of unlabeled exchange partner to the bolus results in a linear increase in rate, absolute values for exchange rate constant (Kexchange) can be determined in both directions. In this study we show that such conditions appear to be true for a bolus of 80mM pyruvate and 40 mM lactate in rat kidney, and use the dynamic imaging data to calculate Kexchange.

Abstract PDF E-Poster Video

Keywords

absolute added addition additional adds appears assumed availability becomes bolus coil comp concentration condition conditions confirms cons containing conversion corrected depends detected determined dire direct disease dissolution dual electrical error every exchange expect fitting flux formation forward good grants health hype ignoring increasing indirect injection injections input just kidney label labeled lactate lead limit limited limiting limits linear made male marker match mayer measure measured metabolic metabolite minus nominal pared park place polarization polarized pool prediction previously produce product production program progression psychiatry quadrature radiology rats resolution response revers reverse rodent sampled saturate saturated sciences simplifies since sodium specificity start starting stead steady stem studied studies switched system tail theory therapy transport typical unchanged unlabeled vein verse volume