Meeting Banner
Abstract #4095

Quantitative in vivo 19F MRI of Transplanted Pancreatic Islets Using Clinically Applicable, Non-Emulsion Nanoparticles

Mangala Srinivas1, Karolina Andralojc2, Andor Veltien3, Maarten Brom2, Leike Joosten2, Arend Heerschap3, Jolanda de Vries1, Carl Figdor1, Otto Boerman2, Mar

1Tumor Immunology, RUNMC, Nijmegen, Gelderland, Netherlands; 2Nuclear Medicine, RUNMC, Nijmegen, Gelderland, Netherlands; 3Radiology, RUNMC, Nijmegen, Gelderland, Netherlands

Pancreatic islet transplant is a promising treatment for type I diabetes. However, islet survival and function is poor post-transplant. Here, we describe a non-emulsion based multimodal agent that is able to penetrate the membrane capsule around islets, and allows for quantitative in vivo 19F MRI, and fluorescence imaging. Labeling had minimal effects on viability and insulin secretion. We present preliminary imaging data in mice that received transplanted, labeled islets. Furthermore, islets remain detectable for at least two weeks post-transplant. Histology confirmed that the label is confined to the transplanted islets. The technique is potentially applicable to longterm, quantitative islet tracking.

Abstract PDF

Keywords

able agent allow allows analyses applicable appropriate blue bore capsule carried cell cells clearly clinical clinically coat coil confirmed confocal consisting constructs contained contrast core customizable describe detect detectable determined development diabetes difficult directly emulsion emulsions encapsulated excised finally fluorescence fluorescent fluorocarbon formulated fragile functional functionality furthermore grants greater health hind hindered horizontal human immunology importantly in vivo instead insulin intact intensity investment islet islets isolated known label labeled labeling lack least limb loaded loading localize long martin media medicine membranous mice microscopy minimal mouse muscle necessary noninvasive novel nuclear nuclei ongoing optical optimal optimized overnight pancreatic part particles patients polymer post primary probe produced promising protocol quadriceps quantification quantitative radiology received recently recipient remain reproducibility required respectively retained review secretion side stability supported surrounded system taken term therapy tissue toxicity tracking transplant transplantation transplanted trends trials tumor type validation various viability viable visible vista volume weeks