Meeting Banner
Abstract #3424

Fibrous Liver Stiffness Analysis Using High Frequency Magnetic Resonance Elastography at 7T on an ex vivo Rat Model. Feasibility and Preliminary Results

Maxime Ronot1, 2, Mathilde Wagner1, Simon Lambert2, Sabrina Doblas2, Ralph Sinkus2, Valerie Vilgrain1, 2, Bernard E. Van-Beers1, 2

1Radiology, Beaujon Hospital, Clichy, France; 2CRB3 INSERM U773, France

The diagnosis, staging and quantification of fibrosis rely on liver biopsy, which is invasive with significant risks. MR Elastography (MRE) is an emerging technique that allows fibrosis assessment by measuring the viscoelastic properties of the liver Our study presents the preliminary results of a high-frequency MRE-assessed fibrous liver analysis in an ex vivo rat model. Fibrosis was induced using CCl4 intoxication. We showed that the mean value of the elasticity shear modulus (Gd) in normal livers was significantly lower than in fibrous ones after 3 weeks of intoxication (p=.01) and correlated to the histological findings. It is expected that Gd will significantly increase in the next 5 weeks.

Keywords

adjacent allow allowing allows alterations always anatomic anesthetized applied around assess assessed beers beginning carbon cellular characterization circular close closely consecutive contiguous correlate correlated correlation developments diameter diluted dose drawn drug dynamics early elasticity evolution example excised excitation expected experimental extracted feasibility fiber fibrosis fibrous findings flexible frequency generated height hence hepatic histological home horizontal hospital humans in vivo included induce induced induction initial injected injection inside intense isotropic light liver livers local located macroscopic made maps measuring mechanical mixture model models modifications modulus molecular motion next normalized olive outside oxygen parametric perspectives phys placed positioning preliminary procedure processed processes propagating propagation proper properties proximity punch quantification quantitatively radiology rats recent reconstructed reconstruction remodeling reported resolution sacrificed sample samples scanner schematic score semi setup shade shaker shear side significantly slice slices sodium spatial specimen spectrometry staining steady step stiffness studies support tissue toothpick transmit transverse twice vibration vibrations view viscoelastic volumetric week weeks