Meeting Banner
Abstract #3055

An Ex-Vivo HR-MAS 1H NMR Metabolic Characterization of APCMin/+ Mouse GI Tumours

Basetti Madhu1, Santiago Uribe Lewis2, Adele Murrell2, John R. Griffiths1

1Molecular Imaging, Cancer Research UK Cambridge Research Institute, Cambridge, England, United Kingdom; 2Epigenetics and Imprinting Laboratory, Cancer Research UK Cambridge Research Institute, Cambridge, England, United Kingdom

The Apc Min/+ mouse is a genetically engineered cancer mouse model that spontaneously develops tumours in the GI tract. Here we have investigated the metabolic characteristics of the GI tumours of Apc Min/+ mice using HRMAS 1H NMR spectroscopy. We found that amino acid metabolism was significantly affected in tumours relative to the normal adjacent tissues. The total choline content was also increased (though not statistically significantly) whereas total creatine remained unchanged. The data show that HRMAS 1H NMR spectroscopy of Apc Min/+ mouse tumour tissues recapitulates some aspects of the metabolic states observed in human tumours.

Abstract PDF E-Poster Video

Keywords

absolute absorbs accumulation acid acids activates adjacent affected animals arch aspects attributed basis bell bile biological biology biopsies brain bred cancer cancers carcinomas carried cell cellular characteristics characterization cholesterol choline clinical collection colon colony common compounds concentrations conjugated conjugation containing content decreased determine develops distal energy engineered england enhanced estimation experiment explanations field formed forming gastrointestinal gene genes genetically glycine human hypo ileum important imprinting in vivo individual institute intermediate investigate investigated investigation kingdom known laboratory lewis like liver maintain maintained malignancy many metabolic metabolism metabolite metabolites methodology mice might model moderate modified molecular mouse mutation neoplasms nucleotide nucleus organs osmotic phantoms phenotype predisposed preliminary previously primarily probe proliferation purine quantified recapitulates reduced reflect regulation remained sample short significantly simulated since software source spectra spectroscopic spectroscopy spin spontaneous spontaneously statistically stress student suggest supported suppressed synthesis tang temperature term though tissue tissues tract unchanged understood unit units usually validated volume water whether widely