Meeting Banner
Abstract #2975

Quantitative 1H HR-MAS Using LC Model Shows Glutamate, Choline, Glycerophosphocholine, and Glucose as Biomarkers of Prostate Cancer

Alan Wright1, May-Britt Tessem2, Helena Bertilsson3, 4, Maria T. Grinde2, Guro F. Giskedegrd2, Jostein Halgunset3, 5, Anders Angelsen4, Arend Heerschap

1Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, Gelderland, Netherlands; 2Department of Circulation and Medical Imaging, NTNU, Trondheim, Norway; 3Department of Laboratory Medicine and Childrens and Womens Health, NTNU, Trondheim, Norway; 4Department of Urology, St Olavs Hospital, Trondheim University Hospital,, Trondheim, Norway; 5Department of Pathology and Medical Genetics, St Olavs Hospital, Trondheim University Hospital, Trondheim, Norway

1H HR-MAS MRS was performed on 131 cores taken from surgically resected prostates from patients with a cancer diagnosis. The cores were characterized with histopathology for the tumour and non-tumour content. LCModel was used with a novel basis set of model metabolites made from careful assignments of a subset of spectra and a formate standard solution for robust and automated quantification procedure. Glutamate, choline and glycerophosphocholine were all found to be positive markers for the presence of tumour tissue, while glucose was significantly reduced in samples containing mostly tumour. Citrate and spermine were found to negatively correlate with the percentage of stroma in benign tissue.

Abstract PDF E-Poster Video

Keywords

accepted accurate accurately added alanine allowed allowing amounts analyses anders applications assignment automated basis benign black bounds cancer cancerous capable careful characterization chemical children choline circulation citrate classified coefficients collect concentration confidence confirmed containing content contents cores corrections correlation cram cylindrical decrease defined detailed example fits formate fully gave generated genetics give gives glucose glutamate glycine health histology hospital important intact knowledge laboratory lactate limit macromolecules made magma maria marker markers measures medical medicine membrane metabolic metabolite metabolites model negative novel optimized pathology pathways patients percentage plots positive previously prior profiles prostate prostates proven pulse quantification quantified quantify quantitative radically radiology reduced reported residual restricted revealed robust routine sample samples selected separate setting several sided significance significantly simulated sins solution spectra spectroscopy spinning statistical strict surgically take thin throughput tissue tool tumor tumorous turn unequal urology useful variances whole width women wright