Meeting Banner
Abstract #1954

Reproducibility of Renal Blood Volume Measurements in Mice

C. Chad Quarles1, Feng Weng1, Mohammed Tantawy1, Rosie Jiang2, Keiko Takahashi2, Chuan-Ming Hao2, Todd Peterson1, Raymond Harris2, Takamune Takahashi2

1Institute of Imaging Science, Vanderbilt University, Nashville, TN, United States; 2O'Brien Mouse Kidney Physiology and Disease Center, Vanderbilt University, Nashville, TN, United States

Renal relative blood volume (RBV) measurements could provide a valuable tool to characterize abnormal renal perfusion in mouse models of kidney disease. We optimized MRI methods for spin-echo based RBV mapping in mouse kidneys and assessed the reproducibility of RBV measurements acquired on consecutive days. The mean kidney RBV measured on consecutive days was 19.97 1.50 and 19.86 1.62, yielding a concordance correlation coefficient of 0.94, indicating that this approach is highly reproducible. Interestingly, the use of a spin-echo sequence preferentially weighted towards microvessels, revealed that microvascular RBV within regions of the medulla were higher than that found in cortex.

Abstract PDF

Keywords

abnormal acquisition agent animals artifacts assays assess assessed assessment avoids blood bottom characterize coefficient comparing complement concordance consecutive contrast correlation cortex cortical course currently days density derived differential disease dose doses dynamic employed employs evaluating excreted facilitate fast filtered function gating gradient herein highly histological hour ideally illustrated improved indicating injection inner institute iron kidney kidneys kinetic known likely major mapping maps matrix measured measures mechanisms medulla mice ming minimize models motion mouse navigator noise optimal optimize optimized outer oxide papilla pathological perfusion physiological physiology preferentially previous rare remained renal repeatability representative reproducibility reproducible resolution respiratory revealed science sensitivity significantly slice spin steady studied studies suited susceptibility tissue tool towards traversing understanding validate validating valuable vary vascular versus vessel vessels volume widely yielding