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Abstract #1801

Analysis of NAA in Gene NAT8l Knock Out Mice Using Proton Magnetic Resonance Spectroscopy

Brian Andrews-Shigaki1, 2, Aryan M.A. Namboodiri3, Asamoah Bosomtwi2, Prasanth Ariyannur3, John Moffett3, Xianling Mao4, Dikoma Shungu4, Reed Selwyn2, Haiying Tang2

1Military & Emergency Medicine, Uniformed Services University of the Health Sciences, Bethesda, MD, United States; 2Radiology & Radiological Sciences, Uniformed Services University of the Health Sciences, Bethesda, MD, United States; 3Anatomy, Phyisiology & Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, United States; 4Department of Radiology, Weill Cornell Medical College, New York, NY, United States

We characterized NAA concentrations in localized brain regions of the NAT8l knockout mouse by using proton magnetic resonance spectroscopy (1H-MRS). Four control and NAT8l knockout mice were scanned on a 7T Bruker scanner using a PRESS sequence. NAA concentrations were significantly decreased in the Cortex and Hypothalamus, with a less significant decrease in the Cerebellum. NAT8l knockout heterozygote mice have significantly decreased NAA levels in the brain. Further studies using these animals will be important for understanding the functional roles of NAA in the brain and its involvement in neurological and mental disorders.

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