Meeting Banner
Abstract #1690

Application of 13C Metabolic Imaging for the Assessment of Ischemia in the Perfused Heart

Daniel Ball1, Rachel Cruickshank1, Carolyn Carr1, Daniel Stuckey2, Kieran Clarke1, Damian Tyler1

1Physiology, Anatomy and Genetics, Oxford University, Oxford, Oxfordshire, United Kingdom; 2Imperial College, London, United Kingdom

The aim of this work was to demonstrate the use of a combined dynamic nuclear polarization and chemical shift imaging protocol in the isolated perfused heart to image metabolism in the setting of both acute and chronic myocardial ischemia. The imaging protocol was successfully applied in both models, and in the acute ischemia model, metabolic disturbances could be clearly visualised. Future work will focus on the translation of these techniques into in vivo models of ischemia.

Abstract PDF

Keywords

acquisition acute advances aerobic allow allows alteration alterations anaerobic anatomy anterior application applied around artery assess assessing assessment ball bicarbonate bore buffer cardiac cardiovascular chemical chronic college combined combining concomitant confirming consistent containing contrast control conversion coronary council decrease demonstrable descending described despite detailed diagnosing disease dissolution dynamic energetic energetics entire examine excised flow foundation fundamental future gadolinium genetics glucose heart hearts heterogeneity identify imperial in vivo increasingly infarct infarction infused initial instruments invasive investigate isolated kingdom lactate lateral magnet male materials matrix medical metabolic metabolism mode model models molecular monitoring myocardial myocardium nuclear nucleus oxford oxidation oxygenated pass perfused perfusion physiology placed plays polarization portion post potential previously process production prognosis proportional protocol rats recent reduced reduction related removed repeated replaced response restrict rodent role sensitivity setting slice spectral spectrum status stitch structure successfully suggest supplied supported switch system therapy tied towards translation ventricle viability weeks whole yielded zone