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Abstract #1651

In Vivo Molecular MRI of GFP/Ferritin Dual Gene Expression

Xiang Nan1, 2, Anna V. Naumova3, Jianmin Wei, Qian Wan4, Lin Gao4, Xin Liu4, Bensheng Qiu, 34

1Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Enginee, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen, Guangdong, China; 2 Inner Mongolia Agricultural University, Huhhot, Inner Mongolia, China; 3Radiology, University of Washington School of Medicine, United States; 4Paul C. Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Enginee, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China

In this study, we aimed to develop a reliable method to monitor the gene expression or therapy in vivo, non-invasively. We first utilized optical imaging to assess EP-mediated transgene expression In Vitro, and then we next constructed a dual mammalian expression vector also carrying a murine ferritin gene, the vector can provide us to monitor the gene expression in vivo by magnetic resonance imaging scanner. DNA was transfected into mouse muscle by electroporation. We succeeded in imaging transgene expression of marine ferritin reporter gene by MR, and further study is going on.

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