Meeting Banner
Abstract #1435

Phosphorus MRS Study of a Murine Model of Peripheral Arterial Disease

Vladimr Mlynrik1, Maxime Pellegrin2, Cristina Cudalbu1, Lucia Mazzolai2, Rolf Gruetter1, 3

1Laboratory of Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland; 2Service of Angiology, University Hospital of Lausanne, Lausanne, Switzerland; 3Departments of Radiology, Universities of Lausanne and Geneva, Switzerland

Diminished perfusion in peripheral arterial disease leads to the development of ischemia, which is thought to initiate angiogenesis, arteriogenesis and enhanced muscle metabolism. Our model of ligating right common iliac artery in mice led to a significant decrease of PCr/γ -ATP measured by localized phosphorus MRS in femoral muscles of the operated leg compared to sham-operated controls one week after surgery. The PCr/γ -ATP ratio was also reduced in the contralateral leg. Five weeks after operation this ratio increased in both legs to levels slightly higher than those in sham-operated animals. There was no significant difference between sedentary and treadmill-exercised animals.

Abstract PDF Poster PDF

Keywords

achieved acknowledgments adjusted alto animals arterial arteries artery atherosclerosis axial blood capacity cell chronic circulating closed coil collected common concentrations containing controls credo decrease denote depends detecting diameter disease dual dynamic energy entirely event events exclusively exercise exercised experimental faster femoral field fitting foundations healthy hind home horizontal hour ideal iliac incision induced inhalation initiate inorganic internal intracellular inversion involves joint junction laboratory laser leading legs ligated ligating limb limbs localization localized loop male measurable measured metabolic metabolism metabolites mice minutes model monitoring morbidity mouse muscle muscles obstruction occurs operated operation oxidative oxygen peak perfusion peripheral phosphate phosphorus position preserved pulses quadrature recovery reduced regeneration repetition rodent saturation sedentary seeking selected selective service sham significantly silk since slice slightly software spectrometer spectroscopic spectroscopy spectrum speed spin started static still supine surface surgery suture therapy tissue training transients treadmill treatment turbo twelve unilateral universities useful vein version view volume water week weeks