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Abstract #0654

Non-Specific Effects in OfMRI: Characterising the fMRI Signal Responses to 445 Nm Light Delivered Via Optic Fibers

SUMMA25Isabel N. Christie1, 2, Jack A. Wells3, Nephtali Marina1, Sergey Kasparov4, Alexander V. Gourine1, Mark F. Lythgoe3

1Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom; 2UCL Centre for Advanced Biomedical Imaging, Division of Medicine and Institute of Child Health, University College London , London, United Kingdom; 3UCL Centre for Advanced Biomedical Imaging, Division of Medicine and Institute of Child Health, University College London, London , United Kingdom; 4School of Physiology and Pharmacology, University of Bristol (IC and JW joint first authors, SK,AG,ML joint senior authors)

This study documents marked non-specific effects of light delivered to the cortex with fMRI. With regard to opMRI such artefacts could confound genuine optogenetically induced hemodynamic changes. Functional MRI responses can be observed using established light delivery protocols, which has profound implications for the design and interpretation of combined optogenetic and MRI experiments.

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