Meeting Banner
Abstract #0268

In Vivo Reduction of Hyperpolarized [1-13C]-Dehydroascorbic Acid Is Affected by Glucose Transporter Expression

SUMMA25Sarah E. Bohndiek1, 2, Mikko I. Kettunen1, 2, David Lewis2, Tiago B. Rodrigues1, 2, Ferdia A. Gallagher1, 2, Dmitry Soloviev2, Kevin M. Brindle1, 2

1Department of Biochemistry, University of Cambridge, Cambridge, Cambridgeshire, United Kingdom; 2Cambridge Research Institute, Cancer Research UK, Cambridge, Cambridgeshire, United Kingdom

Hyperpolarized [1-13C]-Dehydroascorbic Acid (DHA) has recently been demonstrated as a novel imaging biomarker of tumor redox status in vivo. We show here that the rate of [1-13C]-DHA reduction is also influenced by glucose transporter expression, so care should be taken to assess the effect of DHA transport on imaging studies performed with this promising hyperpolarized probe.

Abstract PDF Presentation Video

Keywords

ability according acid acknowledgments activities addition administered administration affected aggressiveness allow alterations although alto analog antioxidant arch around assays assess avid avidly background bearing biochemical biochemistry brindle cancer care caused cell cells chem chemical clamped cohort completion complexes content control correlated culture decline decrease delineated depletion described detectable disease displayed dose drawn drug early environment examined excised explained expression fell form forms freeze funded furthermore give glucose gluts highly horizontal hypothesis hypothesized in vivo indicate indicating induce influence influenced inhibit injected injection institute intensity intracellular invasive kingdom leading lewis little lymphoma magnet membrane mice minutes motivation nature nearly necrosis normalized oxidized panel particular peak phosphate portion post predominantly previously prior prognosis quantified readily readout recently reduced reduction regulation response revealed scale scanner sensing serve shrinkage staining standardized statistically status strongly studies subcutaneous substrate suggest suggesting systems taken threshold tissue tracer transport transported transporter transporters treatment tumor tumors upper uptake verify vitamin volume white