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Abstract #0189

Detecting & Quantifying 2HG & Alterations in Metabolite Profiles in IDH1/2 Mutation Bearing Brain Tumors by Solid State HRMAS NMR

Liya Wang1, Anne Carroll2, Juliya Kalinina3, Erwin Van Meir3, Qiqi Yu1, Junjun Tan2, Ruya Zhao2, Frank Liu4, Shaoxiong Wu4, Hui Mao5

1Radiology, Emory University School of Medicine, Atlanta, GA, United States; 2Chemistry, Emory University, Atlanta, GA, United States; 3Neurosurgery, Emory University School of Medicine, Atlanta, GA, United States; 4NMR Center, Emory University, Atlanta, GA, United States; 5Radiology, Emory University, Atlanta, GA, United States

Solid state high resolution (HR) magic angle spinning (MAS) NMR methods were used to identify the spectroscopic finger print of 2HG in brain tumor tissues to confirm that 2HG is the metabolite marker of IDH mutations. The metabolite profiles of gliomas with and without IDH mutations are investigated quantitatively. The results confirmed that 2HG is a highly specific metabolite marker of IDH mutations in gliomas. In addition, HRMAS NMR analysis provided quantitative metabolite analysis that revealed the altered metabolite profile in tumors bearing with IDH mutations

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Keywords

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