Meeting Banner
Abstract #0164

Tumor Location Is a Major Determinant of Macromolecular Transport, Collagen Fiber Morphology & Metastasis

Marie-France Penet1, Samata Kakkad1, Arvind P. Pathak1, Venu Raman1, Meiyappan Solaiyappan1, Zaver M. Bhujwalla1

1JHU ICMIC Program, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, United States

Prostate cancers growing orthotopically in the prostate result in metastasis to lymph nodes, lungs and liver, and malignant ascites. In contrast, identical xenografts growing heterotopically in the flank rarely result in metastasis. Here we used noninvasive MRI and optical microscopy to characterize interstitial fluid transport and the extracellular matrix in metastasis-permissive or preventive environments using human prostate cancer cells engineered to fluoresce under hypoxia. We observed significant differences in macromolecular transport and collagen I fiber morphology between tumors implanted orthotopically and subcutaneously. These insights may lead to strategies to prevent prostate cancer metastasis.

Abstract PDF Presentation Video

Keywords

administration agent albumin anesthetized animal around avoids believed cancer cells characterization characterize characterized characterizing collagen combined component contrast convection critical density derived described determinant diffusion dimensional dissemination distance distribution drainage draining dynamics early efflux either engineered enhanced environments event excitation expression extracellular exudate fiber fibers flank fluid fluorescent focused geometry gland governing graphs green grown growth harmonic hypoxia identified immunodeficient implantation implanted implanting included influence influx initial injecting inoculation insights intensity inter interstitial intravenous intrinsic invaded kinetics life lobes location lymph lymphatic macromolecular macromolecules magnification maintained major male malignant maps matched matrix metastasis metastasize metastatic mice microscope microscopy mixture morphology node nodes occurred ongoing optical organs overall patients patterns percentage permissive play pooling poor previously primarily profound prognosis progression prostate protein quality quantitative radiology randomly readily relationship representative role school selected several since sites slices stacks studies subcutaneous suturing system tail tissue transport tubular tumor tumors understanding vein view volume volumes