Meeting Banner
Abstract #0023

19F/1H MRI of Brain Inflammation in Experimental Autoimmune Encephalomyelitis

Helmar Waiczies1, 2, Stefano Lepore1, 2, Jason M. Millward3, 4, Bettina Purfrst5, Thoralf Niendorf, 23, Sonia Waiczies1, 2

1Ultrahigh Field Imaging in Neuroinflammation, Experimental and Clinical Research Center (ECRC), a cooperation of the Charit Medical Faculty and the Max Delbrck Center for Molecular Medicine, Berlin, Deutschland, Germany; 2Berlin Ultrahigh Field Facility (B.U.F.F.), Max-Delbrueck-Center for Molecular Medicine, Berlin, Deutschland, Germany; 3Experimental and Clinical Research Center (ECRC), Charit University Medicine Campus Berlin Buch, Berlin, Deutschland, Germany; 4Experimental Neuroimmunology, Charit Universittsmedizin Berlin, , Berlin, Germany; 5Electron Microscopy, Max-Delbrueck-Center for Molecular Medicine, Berlin, Germany

In the present study we employed an animal model of MS, the Experimental Autoimmune Encephalomyelitis (EAE) to explore the in vivo uptake of fluorine (19F) nanoparticles by inflammatory cells during encephalomyleitis. Using a 32-leg 1H/19F birdcage coil dedicated for mouse head imaging, we detected and quantified 19F nanoparticles (containing perfluoro-15-crown-5-ether) taken up and transported by macrophages within the cerebellum following intravenous application. The application of 19F nanoparticles for imaging immune cells in conditions such as encephalomyelitis is an emerging field that will be ideal to study the kinetics of immune cell localization during the development of inflammation.

Abstract PDF Presentation Video

Keywords

acetate administration advantage altered anatomical anesthesia anesthetized animal application around assessed autoimmune background barrier barriers becomes becoming birdcage blood blue body brain bright brooks campus cell cells cerebellum citrate clinical close clustered coil complex constructed containing corresponds crossing cytoplasm daily dedicated dehydration described designed detected detection development diluted disease diseases distinguish distribution dual early electron embedded emerging enclosing engulfed ether examination excitation experimental explored extraction field fluids fluorine free gain geometry gives gradient head histological holder homogeneity human illustrates immune immunized increasingly inflammation inflammatory interference involve iron lesion lesions localization macrophages magma medical medicine mice microscope microscopy model molecular nervous offers onset osmium paramagnetic particles pattern perfused phantom placed predominantly prepared press previously prior process protein recruitment resolved reveal scale scarce sclerosis sections simulated simulations since slice slices smooth software sometimes spectroscopy spheroids stages stained stem step strength studies suppl symptoms system tolerated tracking tunable ultrahigh upon uptake uranyl vascular weighed